Review



hada hydrochloride  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    MedChemExpress hada hydrochloride
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Hada Hydrochloride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA/bio_rxiv__64898__2026__04__14__717102-362-7-9
    Average 94 stars, based on 12 article reviews
    hada hydrochloride - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Pan-cancer circular genomics identifies intratumoral Staphylococcus lugdunensis as a metabolic driver in bladder cancer"

    Article Title: Pan-cancer circular genomics identifies intratumoral Staphylococcus lugdunensis as a metabolic driver in bladder cancer

    Journal: bioRxiv

    doi: 10.64898/2026.04.14.717102

    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote HADA labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Figure Legend Snippet: (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote HADA labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.

    Techniques Used: Infection, Control, Fluorescence, Imaging, Co-Culture Assay, Labeling, Staining, Functional Assay, Protein Concentration, Activity Assay, Two Tailed Test



    Similar Products

    hada hydrochloride  (MedChemExpress)
    94
    MedChemExpress hada hydrochloride
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Hada Hydrochloride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA/bio_rxiv__64898__2026__04__14__717102-362-7-9
    Average 94 stars, based on 1 article reviews
    hada hydrochloride - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    hcc amino d alanine hydrochloride  (MedChemExpress)
    94
    MedChemExpress hcc amino d alanine hydrochloride
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Hcc Amino D Alanine Hydrochloride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA/pm41014824-115-5-8
    Average 94 stars, based on 1 article reviews
    hcc amino d alanine hydrochloride - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    hcc amino d alanine hydrochloride  (Tocris)
    95
    Tocris hcc amino d alanine hydrochloride
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Hcc Amino D Alanine Hydrochloride, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA/pm40299696-307-14-20
    Average 95 stars, based on 1 article reviews
    hcc amino d alanine hydrochloride - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier
    hcc amino d alanine hydrochloride hada  (MedChemExpress)
    94
    MedChemExpress hcc amino d alanine hydrochloride hada
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Hcc Amino D Alanine Hydrochloride Hada, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA+hydrochloride/pm39846310-395-9-13
    Average 94 stars, based on 1 article reviews
    hcc amino d alanine hydrochloride hada - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    red d alanine analogs  (MedChemExpress)
    94
    MedChemExpress red d alanine analogs
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Red D Alanine Analogs, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA+hydrochloride/pmc12091997-234-3-8
    Average 94 stars, based on 1 article reviews
    red d alanine analogs - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    hydrocholoride hada  (MedChemExpress)
    94
    MedChemExpress hydrocholoride hada
    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote <t>HADA</t> labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.
    Hydrocholoride Hada, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hada+hydrochloride/HADA+hydrochloride/pmc10504340-237-7-9
    Average 94 stars, based on 1 article reviews
    hydrocholoride hada - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote HADA labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.

    Journal: bioRxiv

    Article Title: Pan-cancer circular genomics identifies intratumoral Staphylococcus lugdunensis as a metabolic driver in bladder cancer

    doi: 10.64898/2026.04.14.717102

    Figure Lengend Snippet: (A) Heatmap illustrating Spearman rank correlations between bacterial abundance and the infiltration proportions of diverse immune cell populations within the tumor microenvironment. (B) Real time proliferation curves of T24 BLCA cells following exposure to live S. lugdunensis at a multiplicity of infection of 100 to 1 compared to BHI medium control. Data represent the mean plus or minus standard error of the mean (SEM). (C) Representative confocal fluorescence imaging of T24 tumor cells following co culture with S. lugdunensis . Green fluorescent signals denote HADA labeled S. lugdunensis , which were observed localized within malignant cells visualized by red fluorescent actin tracker staining. (D) Volcano plot showing predicted bacterial metagenomic functional pathways and modules via PICRUSt2. (E and F) Volcano plots of differentially abundant lipid metabolites identified through targeted lipidomics, comparing S. lugdunensis lysates versus BHI medium (E) and S. lugdunensis positive tumors versus NATs (F). (G) Venn diagram depicting the intersection of enriched lipid species between bacterial lysates and tumor tissues, identifying LPC14:0 as a candidate effector molecule. (H) Normalized abundance of LPC14:0 in S. lugdunensis (SL) negative and positive tumor tissues. (I) Box plots showing the accelerated proliferation of T24 cells treated with exogenous LPC14:0 relative to vehicle control (six replicates/group). (J) Functional enrichment analysis of differentially expressed genes reveal that the PPAR signaling pathway was significantly enriched in the LPC14:0 treatment group. (K) Quantitative assessment of cellular fatty acid uptake in T24 cells following treatment with vehicle (NC) or 30 µM LPC14:0 for 18 hours. Data are expressed as relative background-subtracted fluorescence intensity (ΔRFU) normalized to total protein concentration (12 replicates/group). (L) Biochemical analysis of FAO activity in T24 cells under the same treatment conditions (six replicates/group). Two-tailed Student’s t -test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; See also Figure S5 and Table S5.

    Article Snippet: The S. lugdunensis strain was stained with HADA hydrochloride (MedChemExpress, Cat# HY-131045, CAS No.: 2253733-10-5) for 30 minutes.

    Techniques: Infection, Control, Fluorescence, Imaging, Co-Culture Assay, Labeling, Staining, Functional Assay, Protein Concentration, Activity Assay, Two Tailed Test